Meet Our Researchers

Evan Macosko

Principal Investigator in the Stanley Center for Psychiatric Research at the Broad Institute of MIT and Harvard
Assistant Professor of Psychiatry at Harvard Medical School
The Question

Our team played a lead role in developing Drop-Seq: a technique for simultaneously profiling thousands of individual cells by separating them into barcoded droplets. Though the method was a significant leap in scale, speed, and affordability over previous, one-cell-at-a-time analyses, it still only allows us to capture data on RNA in the cell. We found ourselves asking: Could we build a system to gather data about all the other kinds of molecules from single cells? Not just RNA, but also DNA, proteins, metabolites, and other molecules?

The Approach

We’re going to use gel balls—technology developed by the Broad’s Fei Chen— to stabilize many different kinds of molecules in the cell and barcode them. It’s a chance to capture more detailed snapshots of cells and, in particular, how those cells might be different in people with mental illness.